Glycosyltransferase GT1 family: Phylogenetic distribution, substrates coverage, and representative structural features
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Glycosyltransferase GT1 family: Phylogenetic distribution, substrates coverage, and representative structural features
Glycosyltransferases (GTS) is responsible for transferring the group glycosyl donor sugar is turned to the acceptor particular, among enzymes GT1 family has been known for a capacity glycosylation their outstanding against natural products as diverse as glycolipids, flavonoids and macrolides etc. However, they do not have inspection this systematically important family members. In this minireview, all sequences were analyzed phylogenetically GT1 family and GT1 grouping exhibited protein domain patterns depending taxonomy life, reveals many untapped clades GTS.
Phylogenetic analysis further characterized GTS facilitate classification GT1 family coverage enzyme substrate of a different life domains, where the GTS of bacteria can tolerate a broader spectrum of chemical skeleton as a substrate, indicate promiscuity is higher than that of other domains. In addition, the size of the order of GTS from a different domain than to understand their different substrate selectivity. Based on multiple sequence alignments of 28 enzymes GT1 representative crystal structure, two important regions located in the N-terminal GTS is identified, the most variable between sequences from different taxonomic domain and is important for substrate binding and / or catalysis.
The key role of these two regions are validated by enumerating the influential residues that interact with the substrate in a representative structure of bacteria and plants. Atlas for family GT1 in terms of phylogeny, substrate selectivity, long sequence, and the critical motif provide clues to discover an unknown GT1s and rational engineering of enzymes known, synthesize glycoconjugates new promise for pharmaceutical applications.
Glycosyltransferase GT1 family: Phylogenetic distribution, substrates coverage, and representative structural features
Structural and Functional Insight Into the Glycosylation Impact Upon HGF / c-Met Signaling Pathway
After a specific interaction with its ligand hepatocyte growth factor (HGF), c-Met signal is relayed to a series of downstream pathways, exert important biological role. Dysregulation of the HGF-c-Met signaling pathway has been involved in the onset, progression and metastasis of various cancers, making the HGF-c-Met axis promising therapeutic target. Both c-Met and HGF undergo glycosylation, which apparently biologically relevant to their function and structural integrity.
Various types of glycoconjugates in the local cellular environment can also set the HGF / c-Met signaling by different mechanisms. However, detailed knowledge related to the glycosylation machinery of HGF-c-Met axis as well as potential applications in oncology research has not been established. Mini review highlights the importance of HGF-c-Met signaling pathway in the context of physiological and pathological, and discuss the molecular mechanisms by which affect glycosylation of HGF-c-Met axis.
Because of the important role played by glycosylation in regulation of the activity of HGF / c-Met, a better understanding of this field is underused may contribute to the development of new therapies that target glycoepitopes. Selective activation of ‘armed’ and ” stripped ‘glycal donors enables stereo glycosylations controlled by using Cu (ii) -catalyst as a promoter has been realized. Results of a typical stereochemistry in this process is mainly influenced by the presence of protecting diverse groups in the donor and solvent system used.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 1-2 mg BSA from Bioprocessed material), 2 ml aff column
Description: N-Acetylglucosamine-6-Sulfatase is a member of the Sulfatase family. N-Acetylglucosamine-6-Sulfatase is required for the lysosomal degradation of the Glycosaminoglycans (GAG) Heparan Sulfate and Keratan Sulfate. N-Acetylglucosamine-6-Sulfatase hydrolyzes the 6-Sulfate groups of the N-Acetyl-D-Glucosamine 6-Sulfate units of Heparan Sulfate and Keratan Sulfate. N-Acetylglucosamine-6-Sulfatase binds 1 Calcium ion per subunit. N-Acetylglucosamine-6-Sulfatase deficiency are the cause of Mucopolysaccharidosis Type 3D (MPS3D), an inborn error leading to lysosomal accumulation of heparan sulfate. MPS3D has profound mental deterioration, hyperactivity, and relatively mild somatic manifestations.
Description: NAGK Human Recombinant produced in E. coli is a single polypeptide chain containing 367 amino acids (1-344) and having a molecular mass of 39.8kDa.;NAGK is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Human N acetylglucosamine kinase Antibody (Biotin Conjugate)
This protocol is compatible with various aglycone including carbohydrates, amino acids, and natural products for access deoxy-glycosides and glycoconjugates with α-anomeric selectivity high.
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3 peptide (repeat-sequence peptide of the P. berghei circumsporozoite protein, CSP) conjugated with BSA)
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4 peptide (minor repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA)
5 peptide (25-aa, repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA)
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 removal kit (Antibody based aff matrix; sufficient to remove 1-2 mg BSA from Bioprocessed material), 2 ml aff column)
 removal kit (Antibody based aff matrix; sufficient to remove 10-20 mg BSA from Bioprocessed material), 10 ml aff column)
 removal kit (Antibody based aff matrix; sufficient to remove 25-50 mg BSA from Bioprocessed material), 25 ml aff column)
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 for ELISA , Western)
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 standards (1 set) for BCA Optima Protein Assay kit)
 standards (750 ug/ml) for BCA Optima Protein Assay kit)
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 Transferase (OGT) Antibody)
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