Glycosyltransferase GT1 family: Phylogenetic distribution, substrates coverage, and representative structural features
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Glycosyltransferase GT1 family: Phylogenetic distribution, substrates coverage, and representative structural features
Glycosyltransferases (GTS) is responsible for transferring the group glycosyl donor sugar is turned to the acceptor particular, among enzymes GT1 family has been known for a capacity glycosylation their outstanding against natural products as diverse as glycolipids, flavonoids and macrolides etc. However, they do not have inspection this systematically important family members. In this minireview, all sequences were analyzed phylogenetically GT1 family and GT1 grouping exhibited protein domain patterns depending taxonomy life, reveals many untapped clades GTS.
Phylogenetic analysis further characterized GTS facilitate classification GT1 family coverage enzyme substrate of a different life domains, where the GTS of bacteria can tolerate a broader spectrum of chemical skeleton as a substrate, indicate promiscuity is higher than that of other domains. In addition, the size of the order of GTS from a different domain than to understand their different substrate selectivity. Based on multiple sequence alignments of 28 enzymes GT1 representative crystal structure, two important regions located in the N-terminal GTS is identified, the most variable between sequences from different taxonomic domain and is important for substrate binding and / or catalysis.
The key role of these two regions are validated by enumerating the influential residues that interact with the substrate in a representative structure of bacteria and plants. Atlas for family GT1 in terms of phylogeny, substrate selectivity, long sequence, and the critical motif provide clues to discover an unknown GT1s and rational engineering of enzymes known, synthesize glycoconjugates new promise for pharmaceutical applications.
Glycosyltransferase GT1 family: Phylogenetic distribution, substrates coverage, and representative structural features
Structural and Functional Insight Into the Glycosylation Impact Upon HGF / c-Met Signaling Pathway
After a specific interaction with its ligand hepatocyte growth factor (HGF), c-Met signal is relayed to a series of downstream pathways, exert important biological role. Dysregulation of the HGF-c-Met signaling pathway has been involved in the onset, progression and metastasis of various cancers, making the HGF-c-Met axis promising therapeutic target. Both c-Met and HGF undergo glycosylation, which apparently biologically relevant to their function and structural integrity.
Various types of glycoconjugates in the local cellular environment can also set the HGF / c-Met signaling by different mechanisms. However, detailed knowledge related to the glycosylation machinery of HGF-c-Met axis as well as potential applications in oncology research has not been established. Mini review highlights the importance of HGF-c-Met signaling pathway in the context of physiological and pathological, and discuss the molecular mechanisms by which affect glycosylation of HGF-c-Met axis.
Because of the important role played by glycosylation in regulation of the activity of HGF / c-Met, a better understanding of this field is underused may contribute to the development of new therapies that target glycoepitopes. Selective activation of ‘armed’ and ” stripped ‘glycal donors enables stereo glycosylations controlled by using Cu (ii) -catalyst as a promoter has been realized. Results of a typical stereochemistry in this process is mainly influenced by the presence of protecting diverse groups in the donor and solvent system used.
Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylglucosamine
Description: Untagged synthetic panspecies N-Acetylglucosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.
Glycated Bovine Serum Albumin (BSA) with ?-N-Acetylglucosamine
Description: Untagged synthetic panspecies N-Acetylglucosamine BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.
Should the Bovine Serum Albumin (BSA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Bovine Serum Albumin (BSA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: NAGK Human Recombinant produced in E. coli is a single polypeptide chain containing 367 amino acids (1-344) and having a molecular mass of 39.8kDa.;NAGK is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human O-Linked-N-Acetylglucosamine Transferase (OGT) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human O-Linked-N-Acetylglucosamine Transferase (OGT) in Tissue homogenates, cell lysates and other biological fluids.
Human O-Linked-N-Acetylglucosamine Transferase (OGT) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human O-Linked-N-Acetylglucosamine Transferase (OGT) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human O-Linked-N-Acetylglucosamine Transferase (OGT) in Tissue homogenates, cell lysates and other biological fluids.
Human O-Linked-N-Acetylglucosamine Transferase (OGT) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human O-Linked-N-Acetylglucosamine Transferase (OGT) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human O-Linked-N-Acetylglucosamine Transferase (OGT) in Tissue homogenates, cell lysates and other biological fluids.
Human O-Linked-N-Acetylglucosamine Transferase (OGT) ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human O-Linked-N-Acetylglucosamine Transferase (OGT) were tested on 3 different plates, 8 replicates in each plate
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human O-Linked-N-Acetylglucosamine Transferase (OGT) in Tissue homogenates, cell lysates and other biological fluids.
Human O-Linked-N-Acetylglucosamine Transferase (OGT) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human O-Linked-N-Acetylglucosamine Transferase (OGT) in samples from Tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Malondialdehyde (MDA)-BSA Conjugate positive control for Western/ELISA
Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) catalyzes the second step in the formation of the mannose 6-
Description: Quantitative sandwich ELISA for measuring Bovine N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Bovine N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA)
Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) catalyzes the second step in the formation of the mannose 6-
Description: Quantitative sandwich ELISA for measuring Bovine N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Bovine N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA)
Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) catalyzes the second step in the formation of the mannose 6-
Description: Quantitative sandwich ELISA for measuring Bovine N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1)
DPAGT1 is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic r
Description: Quantitative sandwich ELISA for measuring Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1)
DPAGT1 is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic r
Description: Quantitative sandwich ELISA for measuring Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1)
DPAGT1 is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic r
Description: Quantitative sandwich ELISA for measuring Human UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA)
Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. NAGPA encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hyd
Description: Quantitative sandwich ELISA for measuring Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA)
Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. NAGPA encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hyd
Description: Quantitative sandwich ELISA for measuring Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA)
Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. NAGPA encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hyd
Description: Quantitative sandwich ELISA for measuring Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA)
Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. Nagpa encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hyd
Description: Quantitative sandwich ELISA for measuring Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA)
Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. Nagpa encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hyd
Description: Quantitative sandwich ELISA for measuring Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA)
Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. Nagpa encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hyd
Description: Quantitative sandwich ELISA for measuring Mouse N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 20-40 mg BSA from Bioprocessed material), 2 ml aff column
Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 50-100 mg BSA from Bioprocessed material), 5 ml aff column
Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 250-500 mg BSA from Bioprocessed material), 25 ml aff column
The microtiter plate provided in this kit has been pre-coated with an antibody specific to O-Linked-N-Acetylglucosamine Transferase (OGT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody
Description: A sandwich ELISA kit for detection of O-Linked-N-Acetylglucosamine Transferase from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A sandwich ELISA for quantitative measurement of Goat Bifunctional UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase(GNE) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat Bifunctional UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase(GNE) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat Bifunctional UDP N acetylglucosamine 2 epimerase/N acetylmannosamine kinase(GNE) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
This protocol is compatible with various aglycone including carbohydrates, amino acids, and natural products for access deoxy-glycosides and glycoconjugates with α-anomeric selectivity high.
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5 peptide (25-aa, repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA)
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 removal kit (Antibody based aff matrix; sufficient to remove 1-2 mg BSA from Bioprocessed material), 2 ml aff column)
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 removal kit (Antibody based aff matrix; sufficient to remove 25-50 mg BSA from Bioprocessed material), 25 ml aff column)
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-BSA Conjugate positive control for Western/ELISA)
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 removal kit (synthetic dye based matrix; sufficient to remove 20-40 mg BSA from Bioprocessed material), 2 ml aff column)
 removal kit (synthetic dye based matrix; sufficient to remove 50-100 mg BSA from Bioprocessed material), 5 ml aff column)
 removal kit (synthetic dye based matrix; sufficient to remove 250-500 mg BSA from Bioprocessed material), 25 ml aff column)
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