Glycosyltransferase GT1 family: Phylogenetic distribution, substrates coverage, and representative structural features

Glycosyltransferases (GTS) is responsible for transferring the group glycosyl donor sugar is turned to the acceptor particular, among enzymes GT1 family has been known for a capacity glycosylation their outstanding against natural products as diverse as glycolipids, flavonoids and macrolides etc. However, they do not have inspection this systematically important family members. In this minireview, all sequences were analyzed phylogenetically GT1 family and GT1 grouping exhibited protein domain patterns depending taxonomy life, reveals many untapped clades GTS.

Phylogenetic analysis further characterized GTS facilitate classification GT1 family coverage enzyme substrate of a different life domains, where the GTS of bacteria can tolerate a broader spectrum of chemical skeleton as a substrate, indicate promiscuity is higher than that of other domains. In addition, the size of the order of GTS from a different domain than to understand their different substrate selectivity. Based on multiple sequence alignments of 28 enzymes GT1 representative crystal structure, two important regions located in the N-terminal GTS is identified, the most variable between sequences from different taxonomic domain and is important for substrate binding and / or catalysis.

The key role of these two regions are validated by enumerating the influential residues that interact with the substrate in a representative structure of bacteria and plants. Atlas for family GT1 in terms of phylogeny, substrate selectivity, long sequence, and the critical motif provide clues to discover an unknown GT1s and rational engineering of enzymes known, synthesize glycoconjugates new promise for pharmaceutical applications.

 Glycosyltransferase GT1 family: Phylogenetic distribution, substrates coverage, and representative structural features
Glycosyltransferase GT1 family: Phylogenetic distribution, substrates coverage, and representative structural features

Structural and Functional Insight Into the Glycosylation Impact Upon HGF / c-Met Signaling Pathway

After a specific interaction with its ligand hepatocyte growth factor (HGF), c-Met signal is relayed to a series of downstream pathways, exert important biological role. Dysregulation of the HGF-c-Met signaling pathway has been involved in the onset, progression and metastasis of various cancers, making the HGF-c-Met axis promising therapeutic target. Both c-Met and HGF undergo glycosylation, which apparently biologically relevant to their function and structural integrity.

Various types of glycoconjugates in the local cellular environment can also set the HGF / c-Met signaling by different mechanisms. However, detailed knowledge related to the glycosylation machinery of HGF-c-Met axis as well as potential applications in oncology research has not been established. Mini review highlights the importance of HGF-c-Met signaling pathway in the context of physiological and pathological, and discuss the molecular mechanisms by which affect glycosylation of HGF-c-Met axis.

Because of the important role played by glycosylation in regulation of the activity of HGF / c-Met, a better understanding of this field is underused may contribute to the development of new therapies that target glycoepitopes. Selective activation of ‘armed’ and ” stripped ‘glycal donors enables stereo glycosylations controlled by using Cu (ii) -catalyst as a promoter has been realized. Results of a typical stereochemistry in this process is mainly influenced by the presence of protecting diverse groups in the donor and solvent system used.

Dinitrophenyl (DNP)-BSA protein Conjugate

AV-9330-BSA 10 mg
EUR 286

anti-N acetylglucosamine kinase

YF-PA26346 50 ul
EUR 334
Description: Mouse polyclonal to N acetylglucosamine kinase

mPEG-BSA (Molecular Weight: 5,000-linear)

PEG-BSA-05K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 10,000-linear)

PEG-BSA-10K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 20,000-linear)

PEG-BSA-20K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 40,000-linear)

PEG-BSA-40K 100 ug
EUR 286

Phosphoserine-BSA control protein

PSER15-N 100 ug
EUR 164

Human N acetylglucosamine kinase Antibody

33313-05111 150 ug
EUR 261

N-Acetylglucosamine Kinase Protein (Recombinant)

20-abx073461
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Anti-N acetylglucosamine kinase (1H8)

YF-MA18793 100 ug
EUR 363
Description: Mouse monoclonal to N acetylglucosamine kinase

(PPPPNAND)3 peptide (repeat-sequence peptide of the P. berghei circumsporozoite protein, CSP) conjugated with BSA

PPPP321-BSA 0.5 mg
EUR 529

(DRAAGQPAG)3 peptide (repeat-sequence peptide of the P. vivax circumsporozoite protein, CSP) conjugated with BSA

DRAA31-BSA 0.5 mg
EUR 529

Bacillus subtilis UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG)

1-CSB-EP330738BRJ
  • EUR 505.00
  • EUR 265.00
  • EUR 1827.00
  • EUR 766.00
  • EUR 1218.00
  • EUR 335.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Bacillus subtilis UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase(murG) expressed in E.coli

(NVDP)4 peptide (minor repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA

NVDP41-BSA 0.5 mg
EUR 529

BSA-N-Acetylglucosamine coated plates for lectin testing by ELISA, 5 plates/pk

NAG16-CP 1 Pk
EUR 408

(NANP)5 peptide (25-aa, repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA

NANP51-BSA 0.5 mg
EUR 529

Rabbit UDP- N- acetylglucosamine- - peptide N- acetylglucosaminy

ELI-23343Ra 96 Tests
EUR 928

Porcine UDP- N- acetylglucosamine- - peptide N- acetylglucosamin

ELI-13296p 96 Tests
EUR 928

Benzoylecgonine-BSA conjugate for ELISA/Western

BNZE15-N 0.5 mg
EUR 164

Bovine Serum Albumin (BSA) ELISA Kit

DLR-BSA-Ge-48T 48T
EUR 387
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Serum Albumin (BSA) ELISA Kit

DLR-BSA-Ge-96T 96T
EUR 494
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 1-2 mg BSA from Bioprocessed material), 2 ml aff column

800-302-BSA 1 Kit
EUR 347

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 10-20 mg BSA from Bioprocessed material), 10 ml aff column

800-310-BSA 1 Kit
EUR 895

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 25-50 mg BSA from Bioprocessed material), 25 ml aff column

800-325-BSA 1 Kit
EUR 1450

N-Acetylglucosamine-6-Sulfatase (GNS) Antibody

20-abx116840
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

N-Acetylglucosamine-6-Sulfatase (GNS) Antibody

20-abx321043
  • EUR 439.00
  • EUR 328.00
  • 100 ul
  • 50 ul

N-Acetylglucosamine-6-Sulfatase (GNS) Antibody

20-abx322511
  • EUR 439.00
  • EUR 328.00
  • 100 ul
  • 50 ul

N-Acetylglucosamine-6-Sulfatase (GNS) Antibody

20-abx002834
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul

N-Acetylglucosamine-6-Sulfatase (GNS) Antibody

abx036402-100ug 100 ug
EUR 391

N-Acetylglucosamine-6-Sulfatase (GNS) Antibody

20-abx006987
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

N-Acetylglucosamine-6-Sulfatase (GNS) Antibody

abx032877-400ul 400 ul
EUR 523

N-Acetylglucosamine-6-Sulfatase (GNS) Antibody

abx032877-80l 80 µl
EUR 286

N-Acetylglucosamine-6-Sulfatase (GNS) Antibody

abx233557-100ug 100 ug
EUR 509

N-Acetylglucosamine-6-Sulfatase (GNS) Antibody

20-abx225195
  • EUR 370.00
  • EUR 606.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 50 ul

N-Acetylglucosamine-6-Sulfatase Recombinant Protein

91-325 0.05 mg
EUR 710.75
Description: N-Acetylglucosamine-6-Sulfatase is a member of the Sulfatase family. N-Acetylglucosamine-6-Sulfatase is required for the lysosomal degradation of the Glycosaminoglycans (GAG) Heparan Sulfate and Keratan Sulfate. N-Acetylglucosamine-6-Sulfatase hydrolyzes the 6-Sulfate groups of the N-Acetyl-D-Glucosamine 6-Sulfate units of Heparan Sulfate and Keratan Sulfate. N-Acetylglucosamine-6-Sulfatase binds 1 Calcium ion per subunit. N-Acetylglucosamine-6-Sulfatase deficiency are the cause of Mucopolysaccharidosis Type 3D (MPS3D), an inborn error leading to lysosomal accumulation of heparan sulfate. MPS3D has profound mental deterioration, hyperactivity, and relatively mild somatic manifestations.

General Bovine Serum Albumin (BSA) ELISA Kit

RD-BSA-Ge-48Tests 48 Tests
EUR 527

General Bovine Serum Albumin (BSA) ELISA Kit

RD-BSA-Ge-96Tests 96 Tests
EUR 732

General Bovine Serum Albumin (BSA) ELISA Kit

RDR-BSA-Ge-48Tests 48 Tests
EUR 551

General Bovine Serum Albumin (BSA) ELISA Kit

RDR-BSA-Ge-96Tests 96 Tests
EUR 766

Mouse UDP- N- acetylglucosamine- - dolichyl- phosphate N- acetyl

ELI-27484m 96 Tests
EUR 865

Bovine UDP- N- acetylglucosamine- - dolichyl- phosphate N- acety

ELI-47979b 96 Tests
EUR 928

Human UDP- N- acetylglucosamine- - dolichyl- phosphate N- acetyl

ELI-39007h 96 Tests
EUR 824

Methamphetamine-N [BSA]

DAG422 1mg
EUR 1104

Phosphothreonine-BSA conjugate (blocking antigen) for ELISA, Western

PTHR15-N 100 ug
EUR 225

Phosphotyrosine-BSA conjugate (blocking antigen) for ELISA , Western

PTYR15-N 100 ug
EUR 225

Testosterone-3-CMO-BSA Conjugate

TEST15-N-100 100 ug
EUR 347

Progesterone-11-CME-BSA Conjugate

P411BSA-N-100 100 ug
EUR 225

Morphine-BSA conjugate, high activity

MORP15-N-1 0.5 mg
EUR 164

Morphine-BSA conjugate, low activity

MORP16-N-1 0.5 mg
EUR 164

Dinitrophenyl (DNP)-BSA protein Conjugate

DNP35-N-10 10 mg
EUR 408

Vaccigel Alum adjuvant adsorbed with Bovine Serum Albumin (BSA @1 mg/ml), Vaccine adjuvant

AV-1010-BSA 1 ml
EUR 286

NAGK N-Acetylglucosamine Kinase Human Recombinant Protein

PROTQ9UJ70 Regular: 20ug
EUR 317
Description: NAGK Human Recombinant produced in E. coli is a single polypeptide chain containing 367 amino acids (1-344) and having a molecular mass of 39.8kDa.;NAGK is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Human N acetylglucosamine kinase Antibody (Biotin Conjugate)

33313-05121 150 ug
EUR 369

N-Acetylglucosamine-6-Phosphate Deacetylase (AMDHD2) Antibody

abx036546-100ug 100 ug
EUR 391

N-Acetylglucosamine-6-Phosphate Deacetylase (AMDHD2) Antibody

abx029897-400ul 400 ul
EUR 523

N-Acetylglucosamine-6-Phosphate Deacetylase (AMDHD2) Antibody

abx029897-80l 80 µl
EUR 286

N-acetylglucosamine-6-phosphate deacetylase (AMDHD2) Antibody

20-abx241754
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

N-acetylglucosamine-6-phosphate deacetylase (AMDHD2) Antibody

20-abx211739
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Human N-Acetylglucosamine Kinase(NAGK)ELISA Kit

QY-E04730 96T
EUR 361

Oxycodone-BSA conjugate for ELISA/Western

OXYC15-N-1 0.5 mg
EUR 286

Morphine-BSA conjugate for ELISA/Western

MORP17-N-1 0.5 mg
EUR 164

Glutamate-BSA Protein conjugate (blocking antigen)

GLM23-N-100 100 ug
EUR 250

Glutamine-BSA Protein conjugate (blocking antigen)

GLN55-N-100 100 ug
EUR 250

Glycine-BSA Protein conjugate (blocking antigen)

GLYC52-N-100 100 ug
EUR 250

Ketamine-BSA conjugate for ELISA/Western

KTM15-N-1 0.5 mg
EUR 347

MDMA-BSA conjugate for ELISA/Western

MDMA16-N-1 0.5 mg
EUR 164

Methadone-BSA conjugate for ELISA/Western

METD15-N-1 0.5 mg
EUR 225

Methamphetamine--BSA conjugate for ELISA/Western

METH15-N-1 0.5 mg
EUR 164

Histamine-BSA Protein conjugate (blocking antigen)

HIST12-N-100 100 ug
EUR 286

Amphetamine-BSA conjugate for ELISA/Western

AMPT15-N-500 0.5 mg
EUR 286

Aspartate-BSA Protein conjugate (blocking antigen)

ASP51-N-100 100 ug
EUR 250

Benzodiazepine-BSA conjugate for ELISA/Western

BNZD15-N-1 0.5 mg
EUR 286

Barbital-BSA conjugate for ELISA/Western

BTL15-N-1 0.5 mg
EUR 347

Buprenorphine-BSA conjugate for ELISA/Western

BUNP15-N-1 0.5 mg
EUR 286

Pre-diluted BSA (bovine serum albumin) standards (1 set) for BCA Optima Protein Assay kit

BCA-BSA-1 1 pk
EUR 164

Recombinant human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

P2172 100ug Ask for price
Description: Recombinant protein for human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase

Pre-diluted BSA (bovine serum albumin) standards (750 ug/ml) for BCA Optima Protein Assay kit

BCA-BSA-750 1 ml
EUR 225

BSA and BGG Stock standards (1 vial each @ 2 mg/ml) for BCA Optima Protein Assay kit

BSA-BGG-1 1 pk
EUR 286

Human UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110KDA subunit (OGT)

1-CSB-EP016315HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110KDA subunit(OGT),partial expressed in E.coli

Anti-OGT/O-Linked N-Acetylglucosamine Transferase Antibody

PB9767 100ug/vial
EUR 334

Human GNS/ N-acetylglucosamine-6-sulfatase ELISA Kit

E2914Hu 1 Kit
EUR 605

Human N acetylglucosamine kinase AssayLite Antibody (FITC Conjugate)

33313-05141 150 ug
EUR 428

Human N acetylglucosamine kinase AssayLite Antibody (RPE Conjugate)

33313-05151 150 ug
EUR 428

Human N acetylglucosamine kinase AssayLite Antibody (APC Conjugate)

33313-05161 150 ug
EUR 428

Human N acetylglucosamine kinase AssayLite Antibody (PerCP Conjugate)

33313-05171 150 ug
EUR 471

O-Linked N-Acetylglucosamine (GlcNAc) Transferase (OGT) Antibody

20-abx126290
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

O-Linked N-Acetylglucosamine (GlcNAc) Transferase (OGT) Antibody

20-abx109393
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

N-Acetylglucosamine-1-Phosphotransferase Subunit Gamma (GNPTG) Antibody

abx340082-100ug 100 ug
EUR 391

O-Linked N-Acetylglucosamine (GlcNAc) Transferase (OGT) Antibody

20-abx001621
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

O-Linked N-Acetylglucosamine (GlcNAc) Transferase (OGT) Antibody

20-abx141655
  • EUR 370.00
  • EUR 606.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul

N-Acetylglucosamine-1-Phosphotransferase Subunit Gamma (GNPTG) Antibody

abx030120-400ul 400 ul
EUR 523

N-Acetylglucosamine-1-Phosphotransferase Subunit Gamma (GNPTG) Antibody

abx030120-80l 80 µl
EUR 286

This protocol is compatible with various aglycone including carbohydrates, amino acids, and natural products for access deoxy-glycosides and glycoconjugates with α-anomeric selectivity high.