Comprehensive structural glycomic characterization of the glycocalyxes of cells and tissues

The glycocalyx consists glycosylation of proteins and lipids and fcorms outermost layer of cells. It is involved in the process of inter- and intracellular fundamentals, including non-self cells and self-recognition, cell signaling, cell structure maintenance, and protective immunity. Characterization glycocalyx is therefore important to understand the physiology of the cell and elucidating its role in promoting health and disease. This protocol describes how to comprehensively characterize glycocalyx N-glycans and O-glycans of glycoproteins and glycolipids intact in parallel, using the same enriched membrane fraction.

Profile of glycans and glycolipids performed using nanoflow liquid chromatography-mass spectrometry (nanoLC-MS). Sample preparation, quantitative LC-tandem MS (LC-MS / MS) analysis, and data processing method is provided. In addition, we discuss the results of the analysis glycoproteomic that specific glycosylation of membrane proteins. To reduce the number of samples required, the N-glycan, O-glycan and glycolipid analysis performed on the same enriched fraction, whereas glycoproteomic analysis performed on a separate enriched fraction. Sample preparation process takes 2-3 d, while the time spent on instrumental analysis and data that can vary from 1 to 5 d for different sample sizes.

This workflow applies to both cell and tissue samples. systematic changes in the glycocalyx associated with certain glycoforms and glycoconjugates can be monitored by quantitation using this protocol. The ability to quantitate individual glycoforms and glycoconjugates will find utility in a variety of basic and applied clinical studies, including glycan-based biomarker discovery and therapy. congenital disorders of glycosylation (CDG) is caused by a defect in a gene that regulates various glycoconjugate biosynthesis. Some of the genes responsible have been identified in the process of N-glycosylation of proteins.

Analysis of mucin-type core-1 O-glycoforms of apolipoprotein C-III (apoCIII) has recently been revealed combined N- and O-glycosylation defects. We apply the matrix-assisted laser desorption / ionization mass spectrometry profile of glycoforms apoCIII 500 serum samples for screening CDG, and the reference values ​​are determined.The contents apoCIII unglycosylated lower in early infancy, showed that the O-glycan occupancy should be judged based reference values ​​of the same age.

 Comprehensive structural glycomic characterization of the glycocalyxes of cells and tissues
Comprehensive structural glycomic characterization of the glycocalyxes of cells and tissues

An important function of glycans in Human Skin Model epithelium dissected by CRISPR-Cas9-Engineered Human Organotypic

glycome change characteristics during histogenesis and organogenesis, but our understanding of the importance of glycan structures selected for tissue formation and homeostasis incomplete.

Here, we present a human Organotypic platform that enables the genetic dissection of cellular glycosylation capacity and interrogation of the role of different types of glycans in tissue formation. We use CRISPR-Cas9 gene targeting to generate a 3D library of tissue selective skin Organotypic differ in their capacity to generate glycan structures on the main types of N- and O-linked glycoproteins and glycolipids.

D-Galactose (D-Gal) (BSA)

20-abx281009
  • EUR 523.00
  • EUR 244.00
  • EUR 1497.00
  • EUR 606.00
  • EUR 384.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.

Bovine Serum Albumin Biotinylated (Biotin-BSA)

BTN-BSA 5 mg
EUR 286

Ibrutinib drug-Bovine Serum Albumin (BSA) Conjugate

IBT15-BSA 100 ug
EUR 529

Dinitrophenyl (DNP)-BSA protein Conjugate

AV-9330-BSA 10 mg
EUR 286

Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 20-40 mg BSA from Bioprocessed material), 2 ml aff column

800-200-BSA 1 kit
EUR 286

Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 50-100 mg BSA from Bioprocessed material), 5 ml aff column

800-205-BSA 1 kit
EUR 773

Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 250-500 mg BSA from Bioprocessed material), 25 ml aff column

800-225-BSA 1 kit
EUR 2425

Glycated Bovine Serum Albumin (BSA) with ?-Galactose

SPR-203A 0.05 mg
EUR 310
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Galactose

SPR-203B 2x0.05 mg
EUR 457
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Galactose

SPR-203C 4x0.05 mg
EUR 653
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

mPEG-BSA (Molecular Weight: 5,000-linear)

PEG-BSA-05K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 10,000-linear)

PEG-BSA-10K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 20,000-linear)

PEG-BSA-20K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 40,000-linear)

PEG-BSA-40K 100 ug
EUR 286

(DRAAGQPAG)3 peptide (repeat-sequence peptide of the P. vivax circumsporozoite protein, CSP) conjugated with BSA

DRAA31-BSA 0.5 mg
EUR 529

(PPPPNAND)3 peptide (repeat-sequence peptide of the P. berghei circumsporozoite protein, CSP) conjugated with BSA

PPPP321-BSA 0.5 mg
EUR 529

(NVDP)4 peptide (minor repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA

NVDP41-BSA 0.5 mg
EUR 529

(NANP)5 peptide (25-aa, repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA

NANP51-BSA 0.5 mg
EUR 529

Bovine Serum Albumin (BSA) ELISA Kit

DLR-BSA-Ge-48T 48T
EUR 387
  • Should the Bovine Serum Albumin (BSA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Serum Albumin (BSA) ELISA Kit

DLR-BSA-Ge-96T 96T
EUR 494
  • Should the Bovine Serum Albumin (BSA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Lactacystin (Synthetic)

1709-200
EUR 316

Lactacystin (Synthetic)

A2583-.1 100 µg
EUR 150
Description: Lactacystin is a specific and an irreversible inhibitor of proteasome with IC50 value of 4.8 ?M [1].Lactacystin binds to the catalytic subunits of the 20 S proteasome and inhibits all the three peptidase activities of the proteasome, chymotrypsin-like, trypsin-like and caspase-like.

Lactacystin (Synthetic)

A2583-.5 500 µg
EUR 473
Description: Lactacystin is a specific and an irreversible inhibitor of proteasome with IC50 value of 4.8 ?M [1].Lactacystin binds to the catalytic subunits of the 20 S proteasome and inhibits all the three peptidase activities of the proteasome, chymotrypsin-like, trypsin-like and caspase-like.

Lactacystin (Synthetic)

A2583-1 1 mg
EUR 790
Description: Lactacystin is a specific and an irreversible inhibitor of proteasome with IC50 value of 4.8 ?M [1].Lactacystin binds to the catalytic subunits of the 20 S proteasome and inhibits all the three peptidase activities of the proteasome, chymotrypsin-like, trypsin-like and caspase-like.

Resveratrol, synthetic

GP5884-100MG 100 mg
EUR 59

Resveratrol, synthetic

GP5884-1G 1 g
EUR 158

Resveratrol, synthetic

GP5884-250MG 250 mg
EUR 87

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 1-2 mg BSA from Bioprocessed material), 2 ml aff column

800-302-BSA 1 Kit
EUR 347

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 10-20 mg BSA from Bioprocessed material), 10 ml aff column

800-310-BSA 1 Kit
EUR 895

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 25-50 mg BSA from Bioprocessed material), 25 ml aff column

800-325-BSA 1 Kit
EUR 1450

General Bovine Serum Albumin (BSA) ELISA Kit

RDR-BSA-Ge-48Tests 48 Tests
EUR 551

General Bovine Serum Albumin (BSA) ELISA Kit

RDR-BSA-Ge-96Tests 96 Tests
EUR 766

General Bovine Serum Albumin (BSA) ELISA Kit

RD-BSA-Ge-48Tests 48 Tests
EUR 527

General Bovine Serum Albumin (BSA) ELISA Kit

RD-BSA-Ge-96Tests 96 Tests
EUR 732

Vaccigel Alum adjuvant adsorbed with Bovine Serum Albumin (BSA @1 mg/ml), Vaccine adjuvant

AV-1010-BSA 1 ml
EUR 286

Atpenin A5 (Synthetic)

2210-1000
EUR 457

Atpenin A5 (Synthetic)

2210-250
EUR 191

Synthetic pyrethroid insecticides

AT123 1mg
EUR 1368

Synthetic pyrethroid insecticide

AG123 1 mg
EUR 523

Synthetic Neurotensin (NT)

4-SPB203Hu01
  • EUR 368.80
  • EUR 202.00
  • EUR 1108.00
  • EUR 436.00
  • EUR 772.00
  • EUR 310.00
  • EUR 2620.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P30990
  • Buffer composition: PBS, pH 7.4.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 1561.9Da
  • Isoelectric Point: 9.7
Description: Recombinant Human Neurotensin expressed in: E.coli

Synthetic Phytohemagglutinin (PHA)

4-SPB359Ge01
  • EUR 368.80
  • EUR 202.00
  • EUR 1108.00
  • EUR 436.00
  • EUR 772.00
  • EUR 310.00
  • EUR 2620.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Inquire
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): Inquire
  • Isoelectric Point: Inquire
Description: Recombinant Pan-species Phytohemagglutinin expressed in: E.coli

Synthetic Triacylated Lipopeptide

VAdv-Ly0036 1 mg
EUR 1628
Description: Synthetic triacylated lipopeptide, a synthetic lipopeptide and a TLR2/1 agonist vaccine adjuvant.

Pre-diluted BSA (bovine serum albumin) standards (1 set) for BCA Optima Protein Assay kit

BCA-BSA-1 1 pk
EUR 164

D-(+)-Galactose

abx082116-25g 25 g
EUR 189
  • Shipped within 5-10 working days.

D-(+)-Galactose

abx082291-25g 25 g
EUR 203
  • Shipped within 5-10 working days.

D-(+)-Galactose

abx082441-25g 25 g
EUR 189
  • Shipped within 5-10 working days.

D-Galactose

HY-N0210 10mM/1mL
EUR 126

D-Galactose

GAL15-N 10 g
EUR 286

L-(-)-Galactose

GC5727-100MG 100 mg
EUR 110

L-(-)-Galactose

GC5727-250MG 250 mg
EUR 205

L-(-)-Galactose

GC5727-25MG 25 mg
EUR 54

L-(-)-Galactose

GC5727-500MG 500 mg
EUR 341

D-(+)-Galactose

GC7360-100G 100 g
EUR 54

D-(+)-Galactose

GC7360-1KG 1 kg
EUR 150

D-(+)-Galactose

GC7360-250G 250 g
EUR 70

D-(+)-Galactose

GC7360-500G 500 g
EUR 94

D-(+)-Galactose

GC7360-5KG 5 kg
EUR 469

D-Galactose

TBW01610 100mg Ask for price

D-(+)-Galactose

GB0215 25g
EUR 56.96
  • Product category: Biochemicals/Carbohydrates/Sugars

Pre-diluted BSA (bovine serum albumin) standards (750 ug/ml) for BCA Optima Protein Assay kit

BCA-BSA-750 1 ml
EUR 225

BSA and BGG Stock standards (1 vial each @ 2 mg/ml) for BCA Optima Protein Assay kit

BSA-BGG-1 1 pk
EUR 286

BSA-galactose coated plates for lectin testing by ELISA, 5 plates/pk

GAL16-CP 1 Pk
EUR 408

(±)-cistrans-Abscisic acid (synthetic)

A068-100MG 100 mg
EUR 90

(±)-cistrans-Abscisic acid (synthetic)

A068-500MG 500 mg
EUR 243

D-erythro-Sphingosine (synthetic)

B6385-100 100 mg
EUR 342

D-erythro-Sphingosine (synthetic)

B6385-25 25 mg
EUR 160

D-erythro-Sphingosine (synthetic)

B6385-50 50 mg
EUR 244

SOF Synthetic Oviduct Fluid.

IVL05-100ML 100 ml
EUR 78
  • Product line: Animal Cell Culture Media
  • Product family: Bovine IVF Media
Description: Synthetic Oviduct Fluid.

SOF Synthetic Oviduct Fluid.

IVL05-6X100ML 6 x 100 ml
EUR 151
  • Product line: Animal Cell Culture Media
  • Product family: Bovine IVF Media
Description: Synthetic Oviduct Fluid.

Calcitonin, Human (Synthetic peptide)

P1433-1
EUR 185

Calcitonin, Human (Synthetic peptide)

P1433-5
EUR 675

synthetic h Glycated Albumin

SGA 1.0 mg
EUR 782

Synthetic Angiotensin II (AngII)

4-SPA005Mi01
  • EUR 350.88
  • EUR 197.00
  • EUR 1040.80
  • EUR 413.60
  • EUR 727.20
  • EUR 298.00
  • EUR 2452.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Inquire
  • Buffer composition: PBS, pH 7.4.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 1046.2Da
  • Isoelectric Point: 6.7
Description: Recombinant Human, Mouse, Rat Angiotensin II expressed in: E.coli

Synthetic Substance P (SP)

4-SPA393Hu01
  • EUR 413.60
  • EUR 214.00
  • EUR 1276.00
  • EUR 492.00
  • EUR 884.00
  • EUR 340.00
  • EUR 3040.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Inquire
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 1347.6Da
  • Isoelectric Point: 12.4
Description: Recombinant Human Substance P expressed in: E.coli

Synthetic Mucin 1 (MUC1)

4-SPA413Hu02
  • EUR 368.80
  • EUR 202.00
  • EUR 1108.00
  • EUR 436.00
  • EUR 772.00
  • EUR 310.00
  • EUR 2620.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P15941
  • Buffer composition: PBS, pH 7.4.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 3756.1Da
  • Isoelectric Point: 7.3
Description: Recombinant Human Mucin 1 expressed in: E.coli

Synthetic Fibrinopeptide A (FPA)

4-SPB237Hu01
  • EUR 413.60
  • EUR 214.00
  • EUR 1276.00
  • EUR 492.00
  • EUR 884.00
  • EUR 340.00
  • EUR 3040.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Inquire
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): Inquire
  • Isoelectric Point: Inquire
Description: Recombinant Human Synthetic Fibrinopeptide A (FPA) expressed in: E.coli

Synthetic Pancreatic Polypeptide (PP)

4-SPB265Hu01
  • EUR 368.80
  • EUR 202.00
  • EUR 1108.00
  • EUR 436.00
  • EUR 772.00
  • EUR 310.00
  • EUR 2620.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P01298
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): Inquire
  • Isoelectric Point: Inquire
Description: Recombinant Human Pancreatic Polypeptide expressed in: E.coli

Synthetic Apelin 13 (AP13)

4-SPB887Hu01
  • EUR 377.76
  • EUR 204.00
  • EUR 1141.60
  • EUR 447.20
  • EUR 794.40
  • EUR 316.00
  • EUR 2704.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q9ULZ1
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): Inquire
  • Isoelectric Point: Inquire
Description: Recombinant Human Apelin 13 expressed in: E.coli

Monophosphoryl Lipid A (Synthetic)

VAdv-Ly0012-1mg 1 mg
EUR 861
Description: Synthetic Monophosphoryl Lipid A, pure compound, a TLR4-based vaccine adjuvant.

Monophosphoryl Lipid A (Synthetic)

VAdv-Ly0012-5mg 5 mg
EUR 2511
Description: Synthetic Monophosphoryl Lipid A, pure compound, a TLR4-based vaccine adjuvant.

Synthetic Monophosphoryl Lipid A

VAdv-Ly0031 1 mg
EUR 2131
Description: Synthetic monophosphoryl lipid A, a TLR4 agonist vaccine adjuvant.

Synthetic FLAG Octapeptide (FLAG)

4-SPX159Ge01
  • EUR 368.80
  • EUR 202.00
  • EUR 1108.00
  • EUR 436.00
  • EUR 772.00
  • EUR 310.00
  • EUR 2620.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Inquire
  • Buffer composition: PBS, pH 7.4.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): Inquire
  • Isoelectric Point: 4
Description: Recombinant Pan-species FLAG Octapeptide expressed in: E.coli

Synthetic TBEV E Peptide

VAng-Lsx0523-inquire inquire Ask for price
Description: Synthetic TBEV E peptide.

Pre-diluted BSA (bovine serum albumin) standards (250, 125, 62.5, 31.25, and 0.0 ug/ml) for BCA Optima Protein Assay kit

BCA-BSA-2 1 pk
EUR 164

Galactose Assay Kit

55R-1459 100 assays
EUR 740
Description: Assay Kit for detection of Galactose in the research laboratory

Galactose Assay Kit

55R-1463 100 assays
EUR 706
Description: Assay Kit for detection of Galactose in the research laboratory

Galactose Kinase antibody

70R-49778 100 ul
EUR 244
Description: Purified Polyclonal Galactose Kinase antibody

Galactose Kinase Antibody

20-abx009234
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

Galactose Mutarotase (Recombinant)

20-abx073309
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug
  • Shipped within 5-10 working days.

DSPE-PEG-Galactose

abx085184-2kDa1g 2 kDa; 1 g
EUR 1191
  • Shipped within 5-10 working days.

DSPE-PEG-Galactose

abx085184-34kDa1g 3.4 kDa; 1 g
EUR 1191
  • Shipped within 5-10 working days.

DSPE-PEG-Galactose

abx085184-5kDa1g 5 kDa; 1 g
EUR 1191
  • Shipped within 5-10 working days.

NH2-PEG-Galactose

abx085244-2kDa1g 2 kDa; 1 g
EUR 565
  • Shipped within 5-10 working days.

NH2-PEG-Galactose

abx085244-34kDa1g 3.4 kDa; 1 g
EUR 565
  • Shipped within 5-10 working days.

NH2-PEG-Galactose

abx085244-5kDa1g 5 kDa; 1 g
EUR 565
  • Shipped within 5-10 working days.

Galactose Standard, 90UL

C146-90UL 90UL
EUR 123

Galactose Oxidase, 1EA

C147-1EA 1EA
EUR 123

Galactose 1-phosphate

HY-113143 10mg
EUR 108

This network library revealed different changes in skin formation associated with the loss of features for all glycoconjugates tested. Organotypic skin models provide phenotypic cues for different functions of glycoconjugates and serves as a unique resource for further genetic dissection and identification of certain structural features involved. This strategy also applies to other Organotypic network model.