N-glycomic profiling of colorectal cancer according to tumor stage and location

Changes in the glycosylation seen in various types of cancer, including colorectal cancer (CRC). Glycans, the sugar moieties of glycoconjugates, is involved in many important functions relevant to cancer and can be of value as a biomarker.

In this study, we have used mass spectrometry to analyze the N-glycan profile of 35 CRC tissue samples and 10 samples of healthy tissue from patients with non-CRC undergoing surgery for other reasons. Tumor samples were divided into groups depending on the location of the tumor (intestinal right or left) and phase (II or III), while samples of healthy intestine is divided into the right or left.

The level of N-glycan composition and class of neutral and acidic glycans are analyzed in a total of ten different groups. Surprisingly, no significant difference in the level of glycan when all right-and left-sided CRC samples are compared, and some differences (such as the H3N5 abundance neutral N-glycan) visible when the sample is divided by both location and stage. Some significant differences were found in the levels of glycans and glycan class when the phase II and III samples were compared, and these glycans can be of value as a potential new marker of cancer progression.

In order to validate our findings, we analyzed samples of healthy tissue of the colon right and left and did not find a significant difference in the level of one of the glycans are analyzed, confirming that our findings when comparing samples of CRC from the right and left colon was not due to normal variation glycans level between right and left intestines healthy. In addition, the levels of glycans H4N3F1P1 acid, H5N4F1P1, and S1H5N4F1 found changes in certain cancers but gut the location-specific manner, suggesting that the CRC affects the level of glycan in the same way regardless of the location of the tumor.

 N-glycomic profiling of colorectal cancer according to tumor stage and location
N-glycomic profiling of colorectal cancer according to tumor stage and location

Sialylation and bioactivity Protein Reduces Serum Amyloid P antifibrotic in Sera of Patients with Idiopathic Pulmonary Fibrosis

Pulmonary fibrosis is a chronic and usually fatal disorder characterized by the progressive formation of scar tissue such as in the lungs. silicic acid is often found as a terminal sugar on glycoconjugates such extracellular protein glycosylations. Sialidases, also known as neuraminidases, glycoconjugates desialylate. Serum amyloid P (SAP), a serum pentameric glycoprotein which has two sialic acids in each polypeptide, inhibits the differentiation of monocytes into fibrocytes and promote human PBMC to accumulate high levels of extracellular IL-10.

When SAP desialylated with sialidase, the effect of SAP on fibrocyte differentiation and IL-10 were severely hampered accumulation. Interestingly, in patients with pulmonary fibrosis, no increased level of sialidase activity in bronchoalveolar lavage fluid, increased levels of sialidases in the lungs, and decreased levels of SAP in the sera. To elucidate the role of SAP desialylation in pulmonary fibrosis (IPF) the pathogenesis of idiopathic, we purified serum SAP from IPF patients and healthy controls and gauge the extent of sialylation and bioactivity of purified SAP.

Ibrutinib drug-Bovine Serum Albumin (BSA) Conjugate

IBT15-BSA 100 ug
EUR 529

Dinitrophenyl (DNP)-BSA protein Conjugate

AV-9330-BSA 10 mg
EUR 286

mPEG-BSA (Molecular Weight: 5,000-linear)

PEG-BSA-05K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 10,000-linear)

PEG-BSA-10K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 20,000-linear)

PEG-BSA-20K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 40,000-linear)

PEG-BSA-40K 100 ug
EUR 286

Glycated Bovine Serum Albumin (BSA) with ?-Galactose

SPR-203A 0.05 mg
EUR 310
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Galactose

SPR-203B 2x0.05 mg
EUR 457
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

Glycated Bovine Serum Albumin (BSA) with ?-Galactose

SPR-203C 4x0.05 mg
EUR 653
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.

(PPPPNAND)3 peptide (repeat-sequence peptide of the P. berghei circumsporozoite protein, CSP) conjugated with BSA

PPPP321-BSA 0.5 mg
EUR 529

(DRAAGQPAG)3 peptide (repeat-sequence peptide of the P. vivax circumsporozoite protein, CSP) conjugated with BSA

DRAA31-BSA 0.5 mg
EUR 529

(NVDP)4 peptide (minor repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA

NVDP41-BSA 0.5 mg
EUR 529

(NANP)5 peptide (25-aa, repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA

NANP51-BSA 0.5 mg
EUR 529

Bovine Serum Albumin (BSA) ELISA Kit

DLR-BSA-Ge-48T 48T
EUR 387
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Serum Albumin (BSA) ELISA Kit

DLR-BSA-Ge-96T 96T
EUR 494
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 1-2 mg BSA from Bioprocessed material), 2 ml aff column

800-302-BSA 1 Kit
EUR 347

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 10-20 mg BSA from Bioprocessed material), 10 ml aff column

800-310-BSA 1 Kit
EUR 895

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 25-50 mg BSA from Bioprocessed material), 25 ml aff column

800-325-BSA 1 Kit
EUR 1450

General Bovine Serum Albumin (BSA) ELISA Kit

RD-BSA-Ge-48Tests 48 Tests
EUR 527

General Bovine Serum Albumin (BSA) ELISA Kit

RD-BSA-Ge-96Tests 96 Tests
EUR 732

General Bovine Serum Albumin (BSA) ELISA Kit

RDR-BSA-Ge-48Tests 48 Tests
EUR 551

General Bovine Serum Albumin (BSA) ELISA Kit

RDR-BSA-Ge-96Tests 96 Tests
EUR 766

Vaccigel Alum adjuvant adsorbed with Bovine Serum Albumin (BSA @1 mg/ml), Vaccine adjuvant

AV-1010-BSA 1 ml
EUR 286

Pre-diluted BSA (bovine serum albumin) standards (1 set) for BCA Optima Protein Assay kit

BCA-BSA-1 1 pk
EUR 164

Pre-diluted BSA (bovine serum albumin) standards (750 ug/ml) for BCA Optima Protein Assay kit

BCA-BSA-750 1 ml
EUR 225

D-(+)-Galactose

GB0215 25g
EUR 56.96

D-Galactose

HY-N0210 10mM/1mL
EUR 126

L-(-)-Galactose

GC5727-100MG 100 mg
EUR 110

L-(-)-Galactose

GC5727-250MG 250 mg
EUR 205

L-(-)-Galactose

GC5727-25MG 25 mg
EUR 54

L-(-)-Galactose

GC5727-500MG 500 mg
EUR 341

D-(+)-Galactose

GC7360-100G 100 g
EUR 54

D-(+)-Galactose

GC7360-250G 250 g
EUR 70

D-(+)-Galactose

GC7360-500G 500 g
EUR 94

D-(+)-Galactose

GC7360-5KG 5 kg
EUR 469

D-(+)-Galactose

GC7360-1KG 1 kg
EUR 150

D-(+)-Galactose

abx082116-25g 25 g
EUR 189

D-(+)-Galactose

abx082291-25g 25 g
EUR 203

D-(+)-Galactose

abx082441-25g 25 g
EUR 189

D-Galactose

GAL15-N 10 g
EUR 286

D-Galactose

TBW01610 100mg Ask for price

BSA and BGG Stock standards (1 vial each @ 2 mg/ml) for BCA Optima Protein Assay kit

BSA-BGG-1 1 pk
EUR 286

Pre-diluted BSA (bovine serum albumin) standards (250, 125, 62.5, 31.25, and 0.0 ug/ml) for BCA Optima Protein Assay kit

BCA-BSA-2 1 pk
EUR 164

BSA-galactose coated plates for lectin testing by ELISA, 5 plates/pk

GAL16-CP 1 Pk
EUR 408

Galactose Standard, 90UL

C146-90UL 90UL
EUR 123

Galactose Oxidase, 1EA

C147-1EA 1EA
EUR 123

Galactose 1-phosphate

HY-113143 10mg
EUR 108

DSPE-PEG-Galactose

abx085184-2kDa1g 2 kDa; 1 g
EUR 1191

DSPE-PEG-Galactose

abx085184-34kDa1g 3.4 kDa; 1 g
EUR 1191

DSPE-PEG-Galactose

abx085184-5kDa1g 5 kDa; 1 g
EUR 1191

NH2-PEG-Galactose

abx085244-2kDa1g 2 kDa; 1 g
EUR 565

NH2-PEG-Galactose

abx085244-34kDa1g 3.4 kDa; 1 g
EUR 565

NH2-PEG-Galactose

abx085244-5kDa1g 5 kDa; 1 g
EUR 565

Galactose Mutarotase (Recombinant)

20-abx073309
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Galactose Kinase Antibody

20-abx009234
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Galactose Assay Kit

55R-1459 100 assays
EUR 740
Description: Assay Kit for detection of Galactose in the research laboratory

Galactose Assay Kit

55R-1463 100 assays
EUR 706
Description: Assay Kit for detection of Galactose in the research laboratory

Galactose Kinase antibody

70R-49778 100 ul
EUR 244
Description: Purified Polyclonal Galactose Kinase antibody

Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 20-40 mg BSA from Bioprocessed material), 2 ml aff column

800-200-BSA 1 kit
EUR 286

Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 50-100 mg BSA from Bioprocessed material), 5 ml aff column

800-205-BSA 1 kit
EUR 773

Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 250-500 mg BSA from Bioprocessed material), 25 ml aff column

800-225-BSA 1 kit
EUR 2425

Human Galactose-Deficient IgA1(Galactose-Deficient IgA1) ELISA Kit

EH4013 96T
EUR 524.1
Description: Method of detection: Indirect ELISA;Reacts with: Homo sapiens;Sensitivity: 8 ng/ml

2-Deoxy-D-galactose

GC5572-10G 10 g
EUR 341

2-Deoxy-D-galactose

GC5572-1G 1 g
EUR 86

2-Deoxy-D-galactose

GC5572-2G 2 g
EUR 126

2-Deoxy-D-galactose

GC5572-5G 5 g
EUR 221

DSPE-PEG-Galactose,10K

LP096072-10K-1g 1g
EUR 852
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

DSPE-PEG-Galactose,2K

LP096072-2K-1g 1g
EUR 852
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

DSPE-PEG-Galactose,3.4K

LP096072-3.4K-1g 1g
EUR 852
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

DSPE-PEG-Galactose,5K

LP096072-5K-1g 1g
EUR 852
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

NH2-PEG-Galactose,10K

HE005072-10K-1g 1g
EUR 359
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

NH2-PEG-Galactose,2K

HE005072-2K-1g 1g
EUR 359
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

NH2-PEG-Galactose,3.4K

HE005072-3.4K-1g 1g
EUR 359
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

NH2-PEG-Galactose,5K

HE005072-5K-1g 1g
EUR 359
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Acetobromo-Alpha-D-Galactose

abx188579-1kg 1 kg
EUR 2388

PCL(5K)-PEG-Galactose

abx085406-2kDa1g 2 kDa; 1 g
EUR 570

Galactose Kinase Blocking Peptide

20-abx062653
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg

Recombinant Human Galactose Mutarotase

7-02794 5µg Ask for price

Recombinant Human Galactose Mutarotase

7-02795 20µg Ask for price

Recombinant Human Galactose Mutarotase

7-02796 1mg Ask for price

Human Galactose ELISA Kit

QY-E05637 96T
EUR 361

EnzyChrom Galactose Assay Kit

EGAL-100 100
EUR 416
Description: Quantitative determination of galactose by colorimetric (570nm) or fluorimetric (530nm/590nm) methods. Procedure: 20 min. Kit size: 100 tests. Detection limit: 10 µM. Shelf life: 3 months. Shipping: on ice; storage: -20°C.

Galactose Mutarotase Recombinant Protein

91-258 0.05 mg
EUR 710.75
Description: Galactose Mutarotase (GALM) is a cytoplasmic enzyme that belongs to the Aldose Epimerase family. GALM is a Mutarotase that converts alpha-Aldose to the beta -Anomer. GALM is active on D-Glucose, L-Arabinose, D-Xylose, D-Galactose, Maltose and Lactose. GALM may be required for normal Galactose metabolism by maintaining the equilibrium of alpha- and beta - anomers of Galactose.

BSA

31C-CH0114 50 mg
EUR 133
Description: Bovine Serum Albumin for use in the research laboratory

Ibrutinib drug-Human Serum Albumin Conjugate

IBT16-BSA 100 ug
EUR 529

Testosterone 3 BSA Protein (BSA)

abx060856-1mg 1 mg
EUR 578

BSA Control for AGE-BSA

35R-AA007 10 mg
EUR 224
Description: BSA Control for AGE protein (BSA modified), Cat No. 30R-AA007

Native Dactylium dendroides Galactose Oxidase

NATE-0273 150 u
EUR 270

Galactose 1-phosphate Potassium salt

HY-113143A 50mg
EUR 165

PCL(5K)-PEG-Galactose, 2K

HE116072-2K-G G
EUR 359
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.

Galactose-1-Phosphate Uridylyltransferase (Recombinant)

20-abx073963
  • EUR 1609.00
  • EUR 328.00
  • EUR 230.00
  • 100 ug
  • 10 ug
  • 2 µg

D-Galactose (D-Gal) Antibody

20-abx178973
  • EUR 1177.00
  • EUR 578.00
  • 1 mg
  • 200 ug

Galactose Colorimetric/Fluorometric Assay Kit

K2126-100 100 assays
EUR 502

Galactose Colorimetric/Fluorometric Assay Kit

K621-100
EUR 512

Histamine [G-BSA] (G-G-BSA)

DAG3327 1mg
EUR 1234

Cyclic citrullinated peptide-BSA(CCP-BSA)

E64I00802 100ug
EUR 343

BSA Antibody

1-CSB-PA272395
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against BSA. Recognizes BSA from Bovine. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:10000, WB:1:1000-1:5000

BSA Antibody

1-CSB-PA000390
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against BSA. Recognizes BSA from Cow. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:2000-5000

BSA Antibody

1-CSB-PA592874
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against BSA. Recognizes BSA from Bovine. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:10000, WB:1:1000-1:5000

BSA Antigen

IM112 1 ml
EUR 285
Description: BSA Antigen by Cygnus Technologies is available in Europe via Gentaur.

Paromomycin [BSA]

DAG-P2529 1mg
EUR 780

Ciclosporin [BSA]

DAG-WZ017A 1 mg
EUR 1040

Acetaminophen [BSA]

DAG2957 0.5mg
EUR 780

Chloramphenicol [BSA]

DAG2974 1mg
EUR 584

Gentamycin [BSA]

DAG2993 1mg
EUR 715

PCP [BSA]

DAG3015 1 mg
EUR 715

estosterone [BSA]

DAG3023 1mg
EUR 910

Digoxin [BSA]

DAG3043 0.5ml
EUR 715

Methamphetamine [BSA]

DAG3101 1mg
EUR 897

Secobarbital [BSA]

DAG3102 1 mg
EUR 780

Oxazepam [BSA]

DAG3106 1 mg
EUR 897

Carisoprodol [BSA]

DAG3107 1 mg
EUR 780

Biotin [BSA]

DAG3197 1mg
EUR 481

Acrolein [BSA]

DAG3266 100ug
EUR 1040

Agmatine [BSA]

DAG3267 100ug
EUR 845

Carnosine [BSA]

DAG3279 1mg
EUR 1105

Diclofenac [BSA]

DAG3299 1mg
EUR 1105

Dopamine [BSA]

DAG3302 1mg
EUR 1105

Adrenaline [BSA]

DAG3306 1mg
EUR 1364

Glycine [BSA]

DAG3313 250ug
EUR 715

Glyoxal [BSA]

DAG3314 250ug
EUR 780

Ibuprofen [BSA]

DAG3353 1mg
EUR 1221

Malondialdehyde [BSA]

DAG3366 1mg
EUR 1170

Naproxen [BSA]

DAG3370 250ug
EUR 585

Nicotinamide [BSA]

DAG3371 1mg
EUR 845

Pyridoxal [BSA]

DAG3394 1mg
EUR 1235

Pyridoxine [BSA]

DAG3395 1mg
EUR 1235

Riboflavin [BSA]

DAG3399 1mg
EUR 845

Rotenone [BSA]

DAG3400 mg
EUR 780

Buprenorphine [BSA]

DAG394 1mg
EUR 780

Propoxyphene [BSA]

DAG397 1 mg
EUR 1105

Fentanyl [BSA]

DAG398 1 mg
EUR 697

Tramadol [BSA]

DAG399 1mg
EUR 1222

Ketamine [BSA]

DAG400 1mg
EUR 715

Cotinine [BSA]

DAG401 1mg
EUR 780

THC [BSA]

DAG406 1mg
EUR 715

Phenobarbital [BSA]

DAG421 1mg
EUR 845

Theophylline [BSA]

DAG426 10mg
EUR 780

Amoxicillin [BSA]

DAG4461 0.5mg
EUR 780

Ampicillin [BSA]

DAG4462 0.5mg
EUR 780

Apramycin [BSA]

DAG4463 0.5mg
EUR 780

Bacitracin [BSA]

DAG4464 1mg
EUR 780

Cloxacillin [BSA]

DAG4467 0.5mg
EUR 780

Neomycin [BSA]

DAG4470 0.5ml
EUR 780

Streptomycin [BSA]

DAG4472 0.5ml
EUR 780

Melamine [BSA]

DAG4476 1mg
EUR 780

Ractopamine [BSA]

DAGA-001B 1mg
EUR 715

Salbutamol [BSA]

DAGA-002B 1mg
EUR 780

Clenbuterol [BSA]

DAGA-003B 1mg
EUR 780

Pb2+ [BSA]

DAGA-005B 1mg
EUR 1105

Cd2+ [BSA]

DAGA-006B 1mg
EUR 897

Hg2+ [BSA]

DAGA-007B 1mg
EUR 897

Cu2+ [BSA]

DAGA-008B 1mg
EUR 780

Ochratoxin [BSA]

DAGA-012B 1mg
EUR 780

Fumonisin [BSA]

DAGA-014B 1mg
EUR 780

Deoxynivalenol [BSA]

DAGA-016B 1mg
EUR 715

Sterigmatocystin [BSA]

DAGA-017B 1mg
EUR 845

Methyltestosterone [BSA]

DAGA-020B 1mg
EUR 780

Medroxyprogesterone [BSA]

DAGA-021B 1mg
EUR 780

Diethylstilbestrol [BSA]

DAGA-022B 1mg
EUR 714

Hexoestrolum [BSA]

DAGA-023B 1mg
EUR 702

Hydrocortisone [BSA]

DAGA-024B 1mg
EUR 780

Estradiol [BSA]

DAGA-025B 1mg
EUR 715

Nonylphenol [BSA]

DAGA-029B 1mg
EUR 780

Ribavirin [BSA]

DAGA-030B 1mg
EUR 1222

Amantadine [BSA]

DAGA-031B 1mg
EUR 780

Sulfadiazine [BSA]

DAGA-032B 1mg
EUR 780

Sulfaquinoxaline [BSA]

DAGA-033B 1mg
EUR 715

Sulfamethazine [BSA]

DAGA-034B 1mg
EUR 780

Sulfadimethoxine [BSA]

DAGA-035B 1mg
EUR 845

Trimethoprim [BSA]

DAGA-036B 1mg
EUR 832

Florfenicol [BSA]

DAGA-039B 1mg
EUR 715

Kanamycin [BSA]

DAGA-040B 1mg
EUR 715

Lincomycin [BSA]

DAGA-042B 1mg
EUR 780

Pirlimycin [BSA]

DAGA-043B 1mg
EUR 780

We found that some IPF patients have abnormally high levels of NEU3 sialidase in their sera and that the SAP in the sera of patients with IPF have abnormally high levels of abnormally low desialylation and the ability to inhibit fibrocyte differentiation and induces accumulation of extracellular IL-10 by PBMCs. These results indicate that SAP desialylation may play a role in the pathogenesis of IPF and which inhibits NEU3 could be a potential therapeutic target for IPF.