Glycan-Functionalized Collagen Hydrogels Modulate the Glycoenvironment of a Neuronal Primary Culture
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Glycan-Functionalized Collagen Hydrogels Modulate the Glycoenvironment of a Neuronal Primary Culture
Glycans play a central role in the development and homeostasis of the central nervous system (CNS), so that changes in the glycosylation profile of the cell surface and extracellular matrix components (ECM) is clear in CNS disorders. based treatment strategy using the regenerative biomaterials platform is being increasingly used to target the disease and to study the glyco-mark of physiological and pathological conditions, especially through the use of biomaterials able to recapitulate the natural components and the physical organization of the ECM. Collagen hydrogel has shown potential as a vehicle for the delivery of cells into the brain, and their therapeutic effect can be expanded by functionalizing them to cope with altered glycosylation patterns with certain brain disorders.
The purpose of this study was to develop and optimize the glycan-functionalized tridimensional collagen-based hydrogels that will make contact with and modulate the differentiation of primary neuronal cultures. Advanced system will provide more information about how the glyco-engineered materials can affect glyco-profile original mark during the process of cell differentiation by modulating the glycan expression differential at the network level.
For this purpose, the collagen polymers undergo a one-step reductive amination with maltose (GLC (α1-4) α-GLC) and lactose (Gal (β1-4) β-GLC) so that the structure can be sacrificed pyranosidic reducing sugars (acting as a linker a) and α-GLC and β-Gal residues exposed, respectively. Biopolymer glycoconjugate used to formulate hydrogels that chemically and biologically characterized, and glyco-profile sign of neuronal cultures after treatment hydrogel was analyzed by lectin staining.
Hydrogel conjugated with glucose limited to 2 weeks astrocytic proliferation and promoted an increase in sialylation while reducing the fucosylation by 2-fold. These results show the differential effect of glycan residues present in the matrix in the expression of cellular sugar and thus has the potential to improve the delivery system of cells in the central nervous system.
Glycan-Functionalized Collagen Hydrogels Modulate the Glycoenvironment of a Neuronal Primary Culture
Simple and Complex Sugars in Parkinson’s disease: a Bitter Taste
neural homeostasis depends on both simple and complex sugars (which glycoconjugates), and their metabolic abnormalities responsible for damaging the nerve function and leads to neurodegeneration. glucose levels increase glycation phenomenon, a wide range of non-enzymatic reactions that cause a variety of intermediates and final products are potentially dangerous in neurons. Glycoconjugates, including glycoproteins, glycolipids, and glycosaminoglycans, contributes to the constitution of the unique features of the membrane and extracellular matrix neurons in the nervous system.
glycosylation defect is often associated with central nervous system disorders and neurodegeneration. Parkinson’s disease (PD) is a neurodegenerative disorder characterized by motor and non-motor symptoms associated with loss of dopaminergic neurons in the substantia nigra pars compacta. Neurons present intracytoplasmic inclusions α-synuclein aggregates are involved in the pathogenesis of the disease together with disorders of autophagy-lysosomal function, oxidative stress, and broken traffic and turnover of membrane components.
Ibrutinib drug-Bovine Serum Albumin (BSA) Conjugate
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.
Glycated Bovine Serum Albumin (BSA) with ?-Galactose
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.
Glycated Bovine Serum Albumin (BSA) with ?-Galactose
Description: Untagged synthetic panspecies Galactose BSA Glycoconjugate for WB, ELISA. The biological activity of this protein has not yet been tested.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 1-2 mg BSA from Bioprocessed material), 2 ml aff column
Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 20-40 mg BSA from Bioprocessed material), 2 ml aff column
Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 50-100 mg BSA from Bioprocessed material), 5 ml aff column
Albumin (Human, Mouse, rat, bovine and others) removal kit (synthetic dye based matrix; sufficient to remove 250-500 mg BSA from Bioprocessed material), 25 ml aff column
Description: Galactose Mutarotase (GALM) is a cytoplasmic enzyme that belongs to the Aldose Epimerase family. GALM is a Mutarotase that converts alpha-Aldose to the beta -Anomer. GALM is active on D-Glucose, L-Arabinose, D-Xylose, D-Galactose, Maltose and Lactose. GALM may be required for normal Galactose metabolism by maintaining the equilibrium of alpha- and beta - anomers of Galactose.
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
Description: A high purity chemical with various applications in medical research, drug-release, nanotechnology and new materials research, cell culture. In the study of ligand, polypeptide synthesis support, a graft polymer compounds, new materials, and polyethylene glycol-modified functional coatings and other aspects of the active compound.
Description: A polyclonal antibody against BSA. Recognizes BSA from Bovine. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:10000, WB:1:1000-1:5000
Description: A polyclonal antibody against BSA. Recognizes BSA from Cow. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:2000-5000
In this review, we chose contributions have recently relevant regarding the direct involvement of glycation and glycosylation stability of α-synuclein, impaired autophagy and function of lysosomes in PD, with a focus on models of potential PD pathogenesis provided by the genetic variants of the enzyme processing glycosphingolipid, especially glucocerebrosidase ( GBA).
